Assassin's Creed Brotherhood is an action-adventure game set in Rome, where Master Assassin Ezio Auditore takes on the Templar Order. Players recruit and command a Brotherhood of Assassins to dismantle the corrupt powers entrenched in the city. The game combines stealth, combat, and leadership as Ezio rallies allies to fight alongside him.

The game also introduces a multiplayer mode to the series, where players choose from a roster of unique characters with distinct weapons and assassination techniques, competing against each other online.